Enzymes and Inhibitors
New England Biolabs, Inc. Bacteroides Heparinase III – 14 units
Bacteroides Heparinase III, also called Heparin Lyase III, is active on both heparin and heparan sulfate. Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
New England Biolabs, Inc. MlyI – 5000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNNN_^.
New England Biolabs, Inc. BstNI – 3000 units
A restriction endonuclease that recognizes the sequence CC^W_GG.
New England Biolabs, Inc. Fnu4HI – 200 units
A restriction endonuclease that recognizes the sequence GC^N_GC.
New England Biolabs, Inc. ClaI – 5000 units
A restriction endonuclease that recognizes the sequence AT^CG_AT.
New England Biolabs, Inc. BbsI – 300 units
A restriction endonuclease that recognizes the sequence GAAGACNN^NNNN_.
Medchemexpress LLC HER2/CD340 Human B 100ug | 100ug
The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (Biotinylated Domain 4 HEK293 His-Avi) is the recombinant human-derived HER2/CD340 protein expressed by HEK293 with C-His-Avi labeled tag
Medchemexpress LLC Gastric lipase protein, human | >95.0% | 10UG
Recombinant human gastric lipase expressed in HEK293 cells and supplied as a His-tagged solution for research use. The enzyme catalyzes hydrolysis of triacylglycerols and is suitable for enzymatic activity assays, biochemical characterization, and reagent validation. Provided as a 10 μg vial with >95% purity; store at -80°C and avoid repeated freeze-thaw cycles.
- Recombinant human enzyme expressed in mammalian (HEK293) cells.
- C-terminal 6xHis tag facilitates purification and detection.
- High purity (>95%) suitable for biochemical and activity assays.
- Complete amino acid sequence available for identity confirmation.
- Stable at -80°C; aliquot to minimize freeze-thaw cycles.
New England Biolabs, Inc. Bacteroides Heparinase II – 200 units
Bacteroides Heparinase II (also called Heparin Lyase II) is cloned from Bacteroides eggerthii. It is a low specificity enzyme that is active on both heparin and heparan sulfate. Bacteroides Heparinase II cleaves the glycosidic bond between N-sulfated and glucuronic or iduronic acid residues. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. When used alone this enzyme rarely yields complete depolymerization of a polysaccharide chain, however disaccharide analysis is enhanced when used in combination with Heparinase I and III.
Cell Biolabs Inc OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit (Colorimetric), 200 assays
Myeloperoxidase (MPO) is a peroxidase enzyme highly expressed in stimuated neutrophil granulocytes. The enzyme has two activities: chlorination and peroxidation. Its chlorination activity produces hypochlorous acid (HOCl), a reactive oxygen intermediate that helps the neutrophils kill microbes. Cell Biolabs' OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, colorimetric assay for measuring the chlorination activity of myeloperoxidase within a sample. Each kit provides sufficient reagents for 200 assays including standards and unknown samples.
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 1000 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.
New England Biolabs, Inc. TaqI Methyltransferase – 1000 units
TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA.
New England Biolabs, Inc. Quick Ligation™ Kit – 150 reactions
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25C)
New England Biolabs, Inc. HincII – 5000 units
A restriction endonuclease that recognizes the sequence GTY_^RAC.
New England Biolabs, Inc. Endo Hf – 100000 units
Endo Hf is a recombinant protein fusion of Endoglycosidase H and maltose binding protein. Endo Hf cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins equally as well as Endo H.